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On-Chip moniToring of Organoids using an oPtoflUidiC systEm

Technological challenge: Health and environment technologies, medical devices (learn more)

Department: Département Microtechnologies pour la Biologie et la Santé (LETI)

Laboratory: Laboratoire Chimie, Capteurs et Biomatériaux

Start Date: 01-10-2021

Location: Grenoble

CEA Code: SL-DRT-22-0707

Contact: charlotte.parent@cea.fr

The thesis is positioned in the domain of organoids, which enable fundamental tissue mechanisms to be studied in-vitro. The project aims at developing a new microfluidic system with integrated simple, robust and compact optical reading, and allowing for in-situ monitoring of the secretome evolution during the cell culture. The chosen model system is the culture of islets of Langerhans responsible for the endocrine function of the pancreas and playing an important role in diabetes. To reach our objective, we propose to combine two approaches within a microfluidic chip: a chip with photonic-crystal sensors that are compatible with lens-less optical reading (LED + CMOS), and a microfluidics technology integrating the pneumatic actuation of an elastic membrane. The main challenges of the project are related to the sensors sensitivity, the optical chip integration in the microfluidic system and the sampling of aliquots without perturbation of the organoid. The thesis will be in collaboration between two complementary laboratory, CEA-LETI (microfluidics, technology), and INL (photonic biosensors).

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