Scientific direction Development of key enabling technologies
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PhD : selection by topics

Engineering science >> Biotechnology, biophotonics
2 proposition(s).

Hyperspectral microscopy and single-shot optical coherence tomography with a static Fourier transform imaging spectrometer

Département Microtechnologies pour la Biologie et la Santé (LETI)

Laboratoire Imagerie et Systèmes d'Acquisition



Fourier Transform Spectroscopy measures the degree of coherence of light to recover the spectrum. A Fourier Transform spectrometer is said static when the fringe pattern is recorded in a single shot with no displacement of mechanical parts. Recently this concept was extended to Hyperspectral Imaging (HSI) for Space applications using a new configuration of static interferometer positioned in front of a focal plane array. Aside from HSI, another possibility that has not yet been investigated is to use this static interferometer for Optical Coherence Tomography (OCT). This PhD project is a collaboration between the Department of Astrophysics of the University of Grenoble and CEA Leti. We propose to investigate this new OCT approach, and its coupling to HSI in a fast bimodal system that could address many applications in Diagnostic and Bioimaging. The student will work on the development of a microscope integrating this new kind of interferometer, as well as the numerical processing of the interference patterns. Applications from students with a solid background in optics and data processing are welcome. A strong interest in biophotonics, and bioimaging is expected.

Raman microspectroscopy coupled to isotopic labelling for the monitoring of antibiotic stress on single bacterial cells

Département Microtechnologies pour la Biologie et la Santé (LETI)

Laboratoire Imagerie et Systèmes d'Acquisition



The antibiogram (antibiotic sensitivity test) test is in the heart of the rationalization of the antibiotic therapies. Faster novel antibiogram methods (answer in less than 2:00) will have a decisive impact in the fight against the extension of the multiresistances of pathogenic bacteria. The current methods, based on the capacity of a molecule to inhibit or not the growth of pathogens, cannot satisfy these speed requirements, because of latency time preliminary to any culture. The Raman microspectroscopy makes it possible to meet this need, with an approach authorizing the characterization of single cells. A time consuming culture, preliminary to the test itself, would not be thus necessary any more. Moreover, the sensitivity to antibiotic would be determined by evaluation of metabolism decrease, a criterion of sensitivity much earlier than growth inhibition. The objective of the thesis is to explore a new method of isotopic marking to measure, thanks to the Raman microspectroscopy, metabolism variations at the level of the single bacterium. Work will implement existing optical devices, as well as techniques of clinical microbiology. Algorithmic developments will concern both spectra and multivariate analysis, and modelling. The candidate must be titular of a diploma of engineer and/or Master degree in signal or data processing, eventually instrumentation, with knowledges in biophysics or optics. Strong interest for microbiology is required.

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