Transfer of knowledge to industry
Département Microtechnologies pour la Biologie et la Santé (LETI)
Laboratoire Systèmes d'Imagerie pour le Vivant
01-03-2020
PsD-DRT-20-0059
At CEA-Leti we have validated a video-lens-free microscopy platform by performing thousands of hours of real-time imaging observing varied cell types and culture conditions (e.g.: primary cells, human stem cells, fibroblasts, endothelial cells, epithelial cells, 2D/3D cell culture, etc.). And we have developed different algorithms to study major cell functions, i.e. cell adhesion and spreading, cell division, cell division orientation, and cell death. The research project of the post-doc is to extend the analysis of the datasets produced by lens-free video microscopy. The post-doc will assist our partner in conducting the experimentations and will develop the necessary algorithms to reconstruct the images of the cell culture in different conditions. In particular, we will challenge the holographic reconstruction algorithms with the possibility to quantify the optical path difference (i.e. the refractive index multiplied by the thickness). Existing algorithms allow to quantify isolated cells. They will be further developed and assessed to quantify the formation of cell stacking in all three dimensions. These algorithms will have no Z-sectioning ability as e.g. confocal microscopy, only the optical path thickness will be measured. We are looking people who have completed a PhD in image processing and/or deep learning with skills in the field of microscopy applied to biology.
Département Métrologie Instrumentation et Information (LIST)
Laboratoire Capteurs et Architectures Electroniques
01-12-2020
PsD-DRT-20-0127
New cancer treatment modalities aim to improve the dose delivered to the tumor while sparing healthy tissue as much as possible. Various approaches are being developed, including the temporal optimization of the dose delivered with very high dose rate irradiation (FLASH). In this particular case, recent studies have shown that FLASH irradiation with electrons was as effective as photon beam treatments for tumor destruction while being less harmful to healthy tissue. For these beams, the instantaneous doses are up to several orders of magnitude higher than those produced by conventional beams. Conventional active dosimeters saturate under irradiation conditions at very high dose rates per pulse, therefore on-line dosimetry of the beam is not possible. We propose to develop a dosimeter dedicated to the measurement of beams in FLASH radiotherapy based on an ultra-fast plastic scintillator coupled with a silicon photomultiplier sensor (SiPM). The novelty of the project lies both in the chemical composition of the plastic scintillator which will be chosen for its response time and its wavelength emission to have a response adapted to the impulse characteristics of the beam, and in the final sensor with the possibility of coupling the plastic scintillator to a miniaturized SiPM matrix. The final goal is to be able to access, with a reliable methodology, the dosimetry and in-line geometry of FLASH beams.
